Electrical stimulation of saccharomyces cerevisiae cultures.Braz. J. Microbiol. [online]. 2004, vol.35, n.1-2 [cited 2015-10-31], pp. 97-103

ABSTRACT
Modulation of cell endogenous membrane potential by an external electrical field influences the structure and function of membrane compartments, proteins and lipid bi-layer. In this work, the effects of applied potential onSaccharomyces cerevisiae growth were characterized through simple yet conclusive experiments. Cell growth time profile and cell division were investigated as macroscopic response to the electrical stimulation. Control experiments were conducted under identical conditions except for the absence of applied potential. Through comparative analysis, electrical stimulation was verified to alter cell cycle as smaller sized population was observed, suggesting that a synchrony in cell division was promoted. Power spectral analysis was employed to sustain synchrony enhancement, and mathematical modeling was conducted for determining kinetic growth changes. Monod type kinetic parameters for growth were determined by non-linear regression. The affinity constant (namely kS) presented a dependence on applied potential suggesting changes on transport across cell membrane. Electrochemically promoted stress was also verified to inhibit growth as well as to induce changes on cell viability.
Key words: electrochemical stress, Saccharomyces cerevisiae, cell cycle, viability.
 



RESULTS AND DISCUSSION
Voltamograms
Steady-state voltamograms were performed in order to evaluate the eletrochemical stability of the YPD medium, i.e. in what range the medium was eletrochemically stable. The obtained voltamograms are shown inFig. 1. No reaction involving glucose electrochemical reactions was detected in the whole voltamogram for three different glucose contents (2%, 10% and 20% w/v), attesting the stability of the main energy source at this potential range.
Additionally, it can be seen that the potential herein applied, +0.75 VSCE, is within the water stability zone. The voltamograms peaks observed in Fig. 1 are due to water decomposition (higher potential) and reduction of dissolved oxygen (lower potential). Thus, important changes on pH are avoided during S. cerevisiaepotentiostatically controlled growth at that potential.
 
 

ARAUJO, Ofelia Q.F. et al. Electrical stimulation of saccharomyces cerevisiae cultures.Braz. J. Microbiol. [online]. 2004, vol.35, n.1-2 [cited  2015-10-31], pp. 97-103 . Available from: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100016&lng=en&nrm=iso>. ISSN 1678-4405.  http://dx.doi.org/10.1590/S1517-83822004000100016.

The Saccharomyces Genome Database (SGD)

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The Saccharomyces Genome Database (SGD) provides comprehensive integrated biological information for the budding yeast Saccharomyces cerevisiae along with search and analysis tools to explore these data, enabling the discovery of functional relationships between sequence and gene products in fungi and higher organisms

http://www.yeastgenome.org

Light travelling from the Sun


Riding Light from Alphonse Swinehart on Vimeo.

Riding Light

from  PLUS  ALL AUDIENCES

 

In our terrestrial view of things, the speed of light seems incredibly fast. But as soon as you view it against the vast distances of the universe, it’s unfortunately very slow. This animation illustrates, in realtime, the journey of a photon of light emitted from the surface of the sun and traveling across a portion of the solar system, from a human perspective.
I’ve taken liberties with certain things like the alignment of planets and asteroids, as well as ignoring the laws of relativity concerning what a photon actually “sees” or how time is experienced at the speed of light, but overall I’ve kept the size and distances of all the objects as accurate as possible. I also decided to end the animation just past Jupiter as I wanted to keep the running length below an hour.
Design & Animation: Alphonse Swinehart / aswinehart.com
Music: Steve Reich “Music for 18 Musicians”
Performed by: Eighth Blackbird / eighthblackbird.org
,

Also See;

Eski Projeler / Old Projects

Naz
(Ben burada yaşarım)

Kendi kendimize karaladığımız ve hoşumuza giden küçük küçük imgeler, insanın varlığını sabitleyebildiği ve toparlanıp odaklayabildiği alanlar açabiliyor. Benim ilgilendiğim karalamaların ortaya çıktığı anların dürüstlüğünü ve kişisel estetiğini bir sürece ve seyirci ilişkisine taşıyabilmek.

Naz, toplumsal beklentiler ve varoluş arasında sıkışmaya ve de sistematik bir izsizleştirmeye incelerek direnir. Kaligrafi fırçaları ve çini mürekkebi ile yaptığım bu seride direniş ve varoluş üzerine soyut çalışmalar yer alıyor.

Sunum panoları, fotokopi kağıtları ve paket kağıtlarının üzerinde resmi ve gayri resmi ilişkiler ve kopukluklar beliriyor, imzalanıyor mühürlenip açılıyor. Takip ve tekrar içerisinde, beyaz üzerine siyah ile çizgi ve lekelerle kurduğum dünyamı izleyiciye açıyorum.

2007, Alphan Vardarlı

Coyness
(I live here)

Little images we scribble to ourselves, can open areas where a person can establish his existence and get oneself together to focus. What I am interested here is to carry the honesty and the personal aesthetics of those moments to a process and a viewer relation.

Coyness resists being congested between social expectations and existence and a systematic cluelessness by appearing more refined. In this series, which I produced with calligraphy brushes and Indian ink, there are abstract works on resistance and existence.

On the office white surfaces of presentation boards and photocopy papers and upon the soft brown of wrapping papers, official and unofficial relations and non relations appear, get signed, sealed and opened. I am opening up my world to the viewers, which I have set up within pursuit and repetition, with black on white, with line and stain.

2007, Alphan Vardarlı

Resim1

Sonsuzluk Çözümü Sergisi – Notlar Bölümü / Infinity Solution Exhibition – Notes Section (2006)

bazen

Resim1

KALİGRAFİK KARALAMALAR

09.2005 Galata Perform “Görünürlük” Projesi

KALİGRAFİK KARALAMALAR
Kaligrafi : harfleri, güzel biçimler vererek yazma sanatı, güzel yazı sanatı.
Karalama : 1 karalamak eylemi. 2 el alıştırmak için yinelene yinelene yazılan yazı. 3 bir yazının, üstünde düzeltmeler yapılan, temize çekilmemiş taslağı. 4 kara çalma, leke sürme, suç yükleme. (A. Püsküllüoğlu, Türkçe Sözlük, Yapı Kredi Yayınları, 1995)
Benim için çok özel bir dünyaya bir gezinti çizdiklerim. Elimi ve hayal gücümü olabildiğince serbest bırakıp, çoğu zaman müziğe kapılıyor ve düzeltmeye silip bozmaya gitmeksizin, sonsuz bir tekrar içinde ittiğim çizgiyi takip ediyorum. Bu sürekliliğin içinde, tekrarlayarak incelen bir estetikte, kendi dilimi bulmaya çalışıyorum. İçlerinde bir alfabe saklı gibi duran sayfalarda bu bazen bir bütünlüğe ulaşıyor; resimleşiyor.
Defterleri matbaalarda kullanılan kitap maketleri oluşturuyor.
Kullandığım markörler ise edding, artline ve de yoken.
Alphan Vardarlı, 2005

Kaligrafik Karalamalar from ALPHAN VARDARLI on Vimeo.

 

Resim1

 

212495

Büyük Boşluk – The Great Emptiness 15 Kasım – 31 Aralık 2005

Kuratör : Marcus Graf

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