Gimzewski Lab – Cell Sounds

Cell Sounds

A. E. Pelling, S.Sehati, E. B. Gralla, J. S. Valentine, and J. K. Gimzewski, “Local Nanomechanical Motion of the Cell Wall of Saccharomyces cerevisiae,” Science, 305, 1147-1150 (2004)
Like passing freight trains that shake the walls of nearby houses, cargo-carrying proteins shake the cell walls of yeast. Gimzewski’s team measured these tiny cell wall vibrations in a species of yeast used to make bread rise. They performed many experiments to try to understand what makes the cell walls vibrate. They think that the protein freight carriers, also called molecular motors, cause the wall vibrations as they transport everything from bits of protein to whole chromosomes throughout the cell.
Since sound is created by vibrations, the scientists also determined how the cell wall movements would sound if they could be heard. If the yeast cell was the size of a human, the sounds it would make would not be like loud music. “They would be more like the sounds of an ordinary conversation,” said Gimzewski. Gimzewski thinks sound is an interesting way to present observations of vibrating cell walls. The movements are too small and fast to be seen on video.
To investigate why the cells vibrate, Gimzewski studied the vibrations at different temperatures. They also treated yeast cells with a chemical that prevents the cells from producing energy. The vibrations stopped when the cells stopped producing energy. From these and a few other experiments and calculations, the scientists say that cell wall motion in yeast is probably caused by the activity of many molecular motors working within the cell at the same time. Gimzewski thinks these movements might be useful to the yeast cells. The motion could be part of a communication pathway or a pumping action that helps move nutrients or chemicals from one side of the cell wall to the other.
 

Electrical stimulation of saccharomyces cerevisiae cultures.Braz. J. Microbiol. [online]. 2004, vol.35, n.1-2 [cited 2015-10-31], pp. 97-103

ABSTRACT
Modulation of cell endogenous membrane potential by an external electrical field influences the structure and function of membrane compartments, proteins and lipid bi-layer. In this work, the effects of applied potential onSaccharomyces cerevisiae growth were characterized through simple yet conclusive experiments. Cell growth time profile and cell division were investigated as macroscopic response to the electrical stimulation. Control experiments were conducted under identical conditions except for the absence of applied potential. Through comparative analysis, electrical stimulation was verified to alter cell cycle as smaller sized population was observed, suggesting that a synchrony in cell division was promoted. Power spectral analysis was employed to sustain synchrony enhancement, and mathematical modeling was conducted for determining kinetic growth changes. Monod type kinetic parameters for growth were determined by non-linear regression. The affinity constant (namely kS) presented a dependence on applied potential suggesting changes on transport across cell membrane. Electrochemically promoted stress was also verified to inhibit growth as well as to induce changes on cell viability.
Key words: electrochemical stress, Saccharomyces cerevisiae, cell cycle, viability.
 



RESULTS AND DISCUSSION
Voltamograms
Steady-state voltamograms were performed in order to evaluate the eletrochemical stability of the YPD medium, i.e. in what range the medium was eletrochemically stable. The obtained voltamograms are shown inFig. 1. No reaction involving glucose electrochemical reactions was detected in the whole voltamogram for three different glucose contents (2%, 10% and 20% w/v), attesting the stability of the main energy source at this potential range.
Additionally, it can be seen that the potential herein applied, +0.75 VSCE, is within the water stability zone. The voltamograms peaks observed in Fig. 1 are due to water decomposition (higher potential) and reduction of dissolved oxygen (lower potential). Thus, important changes on pH are avoided during S. cerevisiaepotentiostatically controlled growth at that potential.
 
 

ARAUJO, Ofelia Q.F. et al. Electrical stimulation of saccharomyces cerevisiae cultures.Braz. J. Microbiol. [online]. 2004, vol.35, n.1-2 [cited  2015-10-31], pp. 97-103 . Available from: <http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100016&lng=en&nrm=iso>. ISSN 1678-4405.  http://dx.doi.org/10.1590/S1517-83822004000100016.

Light travelling from the Sun


Riding Light from Alphonse Swinehart on Vimeo.

Riding Light

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In our terrestrial view of things, the speed of light seems incredibly fast. But as soon as you view it against the vast distances of the universe, it’s unfortunately very slow. This animation illustrates, in realtime, the journey of a photon of light emitted from the surface of the sun and traveling across a portion of the solar system, from a human perspective.
I’ve taken liberties with certain things like the alignment of planets and asteroids, as well as ignoring the laws of relativity concerning what a photon actually “sees” or how time is experienced at the speed of light, but overall I’ve kept the size and distances of all the objects as accurate as possible. I also decided to end the animation just past Jupiter as I wanted to keep the running length below an hour.
Design & Animation: Alphonse Swinehart / aswinehart.com
Music: Steve Reich “Music for 18 Musicians”
Performed by: Eighth Blackbird / eighthblackbird.org
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